Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
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Intramuscular (IM) Injection Procedure
It is optimal for an intramuscular injection to have in possession syringes without the tips (needles) already affixed to them. Preferably, the individual should have the hermetically sealed syringes (barrels) separate from the hermetically sealed needle tips. Although one can easily use syringes with the tips already affixed, it is slightly more complicated and adds an extra step or two into the process that otherwise would not be there. So, every individual should ensure to the best of their ability to have the syringe and needle tips separate. The following is a list of required items for intramuscular injections :